Discovering genes associated with dormancy in the monogonont rotifer Brachionus plicatilis

Background: Microscopic monogonont rotifers, including the euryhaline species Brachionus plicatilis, are typically found in water bodies where environmental factors restrict population growth to short periods lasting days or months. The survival of the population is ensured via the production of resting eggs that show a remarkable tolerance to unfavorable conditions and remain viable for decades. The aim of this study was to generate Expressed Sequence Tags (ESTs) for molecular characterisation of processes associated with the formation of resting eggs, their survival during dormancy and hatching. Results: Four normalized and four subtractive libraries were constructed to provide a resource for rotifer transcriptomics associated with resting-egg formation, storage and hatching. A total of 47,926 sequences were assembled into 18,000 putative transcripts and analyzed using both Blast and GO annotation. About 28-55% (depending on the library) of the clones produced significant matches against the Swissprot and Trembl databases. Genes known to be associated with desiccation tolerance during dormancy in other organisms were identified in the EST libraries. These included genes associated with antioxidant activity, low molecular weight heat shock proteins and Late Embryonic Abundant (LEA) proteins. Real-time PCR confirmed that LEA transcripts, small heat-shock proteins and some antioxidant genes were upregulated in resting eggs, therefore suggesting that desiccation tolerance is a characteristic feature of resting eggs even though they do not necessarily fully desiccate during dormancy. The role of trehalose in resting-egg formation and survival remains unclear since there was no significant difference between resting-egg producing females and amictic females in the expression of the tps-1 gene. In view of the absence of vitellogenin transcripts, matches to lipoprotein lipase proteins suggest that, similar to the situation in dipterans, these proteins may serve as the yolk proteins in rotifers. Conclusion: The 47,926 ESTs expand significantly the current sequence resource of B. plicatilis. It describes, for the first time, genes putatively associated with resting eggs and will serve as a database for future global expression experiments, particularly for the further identification of dormancy related genes. read more

Elevation Certificates in Demand as Flood Insurance Rates Rise

first_imgContractors elevate a home at 10th Street and the bay. Properties above a specified ‘base flood elevation’ will pay far less in flood-insurance premiums under new legislation and knowing an existing property’s elevation becomes increasingly important. Photo credit: John BallIn Ocean City’s bustling real estate market, “location, location, location” has been replaced by a new adage: “elevation, elevation, elevation.”But home buyers are no longer required to produce an elevation certificate to learn the actuarial (full risk) rate of insuring their property against a flood before they close a deal.The April 2014 Homeowner’s Flood Insurance Affordability Act (HFIAA) undid a provision of the 2012 Biggert-Waters Flood Insurance Reform Act that included the elevation certificate requirement.Although that actuarial rate might remain a mystery for those who have recently purchased “pre-FIRM” homes — built prior to the 1975 adoption of the first flood insurance rate map — ignorance is not necessarily bliss.After decades of taxpayer subsidies to bolster the National Flood Insurance Program, now billions of dollars in debt, the HFIAA calls for annual increases in flood insurance premiums until actuarial rates are achieved. Without an elevation certificate it is virtually impossible to know when a homeowner has paid too much, according to Bill McMahon III, president of the McMahon Agency.“A lot of times [a home’s elevation] isn’t as bad as you think.  But you need to know where you are at, you need to know that worst-case scenario, because the rates are only going to go up,” he says.Tom Heist, president of Thomas H. Heist Insurance Agency of Ocean City, says that even owners with no intention of selling their homes can benefit from ordering an elevation certificate.  In addition to specifying the elevation of a home’s first-floor of living space, the certificate documents whether a home has adequate flood venting, its crawlspace grade and the location of mechanicals like central air conditioning condenser units, each of which can dramatically affect premiums.“If we have an elevation certificate on file, we will rate the property both ways — with the elevation certificate and without — and give the owner the lesser of the two,” Heist says. “It won’t be used against you.”While some sellers in Ocean City have been reluctant to obtain an elevation certificate, leaving buyers to foot the bill while in escrow on a home they don’t yet own, Coldwell Banker agent Lauren Perkins says others are taking the initiative.“If you are a smart seller you will find out everything you can about your property before you list it, and that includes the elevation,” Perkins maintains. “Sellers should do their homework up front.  Ultimately, it makes their property more marketable.”The question of which party — buyer or seller — bears responsibility for obtaining an elevation certificate appears to have no clear cut answer.Edward Rogan, a spokesman with the New Jersey Department of Banking and Insurance, which regulates the real estate industry, says that agents are required to “disclose all information material to the physical condition of the property which they know, or which a reasonable effort to obtain such information would have revealed.”FEMA, in a fact-sheet for Realtors, maintains only that “buyers can ask sellers to provide an elevation certificate” but sellers are under no obligation to comply.Adam DeSanctis, a spokesman with the National Association of Realtors, says that elevation certificates — like most home inspection-related issues — are negotiable between a buyer and seller and are “not something that one or the other should be responsible for paying.”Despite that lack of consensus, business has been brisk for Egg Harbor-based land surveyor Jim Boney as warmer temperatures spark home sales along the Jersey Shore.  “People want to know what their insurance liability will be, especially when buying in a coastline area” according to Boney, who estimates that the requests he has fielded have been split “about 50-50” among buyers and sellers.“The seller’s agent is asking, ‘What do I have to do to make this house sellable?’ And so they will get the seller to order an elevation certificate, just so that it’s on hand and it becomes part of their package for a buyer. If they are the buyer’s agent, they will go to their client and say ‘If you’re serious, and you’re interested in this house, order the elevation certificate and incur the cost,’ ” Boney says.“Some agents that are really proactive will actually pay for the elevation certificates themselves,” he adds. “It’s worth it to them if they can move a sale along and get the deal done.”Ocean City Board of Realtors President Gloria Votta notes that the need to order an elevation certificate can often be negated with a quick phone call to McMahon or Heist.  “If they already have one on file for a property, they will usually let us know what the elevation is,” she says.But when no pre-existing elevation certificate can be located, or when a pre-existing elevation certificate has become outdated, Votta believes that buyers should strongly consider ordering it themselves.“It’s too important, too big of a deal, to not know your elevation. If you’re going to invest half-a-million dollars in a house, spend the extra few hundred on the certificate. It’s a small price to pay,” Votta says.  “Especially when not knowing the elevation could cost you more money down the road.”McMahon agrees.“Investing in a shore property is one of the largest assets you’re ever going to have. An elevation certificate is money well spent,” he says.last_img read more

PODCAST: Put your people first

first_img Jason Lindstrom can summarize his best crisis leadership advice in one word: Listen.Lindstrom, president/CEO of $316 million asset Evergreen Credit Union in Portland, Maine, and chair of the CUNA Marketing & Business Development Council, says that means putting the financials aside for a moment and focusing first on your people.In this episode of the CUNA News Podcast, Lindstrom takes us through Evergreen’s response to the COVID-19 crisis, which includes participating in the first wave of Paycheck Protection Program loans, holding a virtual annual meeting, leading a remote workforce, and other challenges. ShareShareSharePrintMailGooglePinterestDiggRedditStumbleuponDeliciousBufferTumblr Jason Lindstromcenter_img continue reading »last_img

Irish Water announce plans to fix Falcarragh water debacle

first_imgEngineers from Irish Water held a constructive meeting with elected officials from the Gortahork and Falcarragh area this morning to discuss the utility’s plans to replace two sections of water pipes that are prone to frequent bursts.Councillors were told that Irish Water is committed to delivering water improvement works in Falcarragh and financial approval has been given to progress this through the National Leakage Reduction Programme.This programme is being delivered across Donegal and the rest of the country to replace pipes and repair leaks, targeting and prioritising investment where it is most needed. Irish Water acknowledged the significant ongoing level of disruption to water supply in the area due to regular bursts on the network.Local county councillor Micheal Mac Giolla Easbuig welcomed the news of the planned upgrade of the system.The existing pipework is asbestos concrete, a common pipe material used in the 1980s which tends to burst quite frequently as the pipes reach the end of their life cycle.Commenting Kevin Love, Irish Water said: “This is not an acceptable situation and we are aware of the impact on local residents and businesses in the area. We are committed to dealing with the issues highlighted as soon as is achievable. Irish Water agrees with the need to replace these pipes as a matter of urgency and we have reprioritised water mains replacement work in Donegal to bring this project to the top of our list. “Until the upgrade works are completed, Donegal County Council, in partnership with Irish Water, will work to minimise disruptions due to future interruptions to supply by working to prevent bursts, prioritising any outages that might occur and carrying out all repairs as quickly as possible.”The Falcarragh project is currently in the design stage of delivery. This week our regional contractor Farran’s Construction Ltd began site survey works required for design.There are two sections of water mains planned for improvement:Replacement of problematic rising mains to the Cashel reservoir with approximately 1.7km of new rising main.Replacement of problematic water mains on the distribution network with 1.4km of new water mains. This will include new service connections from the new water main to each individual property. Associated to the design of an engineering project such as water pipe replacement there are a number of regulatory requirements which must be met in advance of construction commencement.Irish Water currently plans to commence the construction of this project in Autumn of this year.A spokesman added “We will continue to engage with the local community and elected officials once the detailed design is complete and dates for commencement are available.“Communications will also issue during the delivery of the project to ensure the local community are kept informed of progress. “The completion of this water network improvement project will provide the wider community in Falcarragh with a more reliable secure water supply and also reduce leakage.”Irish Water announce plans to fix Falcarragh water debacle was last modified: September 6th, 2019 by StephenShare this:Click to share on Facebook (Opens in new window)Click to share on Twitter (Opens in new window)Click to share on LinkedIn (Opens in new window)Click to share on Reddit (Opens in new window)Click to share on Pocket (Opens in new window)Click to share on Telegram (Opens in new window)Click to share on WhatsApp (Opens in new window)Click to share on Skype (Opens in new window)Click to print (Opens in new window)Tags:FalcarraghIrish Watermeetingpipeslast_img read more

Birmingham ‘ready to sell keeper Foster for £4m’

first_imgBirmingham City are ready to sell Ben Foster for £4m, according to the Daily Mirror.QPR have previously been linked with a £5m bid for the former England international, who is one of several goalkeepers boss Mark Hughes is considering as a possible replacement for Paddy Kenny.Foster spent last season on loan at West Brom, who were hoping to buy him this summer, but Rangers are tipped to sign him.Several papers report the news that John Terry, who has been nursing a hamstring strain, is likely to be fit for England’s Euro 2012 match against France, but his Chelsea team-mate Ashley Cole is struggling with a stomach bug.Meanwhile, The Independent suggest Chelsea have not given up hope of building a new stadium on the Battersea Power Station site despite losing out on preferred bidder status to Malaysian developers.And the Daily Mail say Fulham are interested in St Etienne’s Romanian defender Banel Nicolita.This page is regularly updated. Follow West London Sport on TwitterFind us on Facebooklast_img read more

South African soccer fans 9

first_imgClick on a thumbnail for a low-resolution image, or right-click on the link below it to download a high-resolution copy of the image.  Photo:• Download high-resolution image Photo:• Download high-resolution image Photo:• Download high-resolution image Photo:• Download high-resolution image Photo:• Download high-resolution image Photo:• Download high-resolution image Photo:• Download high-resolution image Photo:• Download high-resolution image Photo:• Download high-resolution image {loadposition fifa}last_img

Microloans available for small to medium sized farms

first_imgShare Facebook Twitter Google + LinkedIn Pinterest Agriculture Deputy Secretary Krysta Harden announced that the U.S. Department of Agriculture (USDA) will begin offering farm ownership microloans, creating a new financing avenue for farmers to buy and improve property. These microloans will be especially helpful to beginning or underserved farmers, U.S. veterans looking for a career in farming, and those who have small and mid-sized farming operations.“Many producers, especially new and underserved farmers, tell us that access to land is one of the biggest challenges they face in establishing and growing their own farming operation,” Harden said. “USDA is making it easier for new farmers to hit the ground running and get access to the land that they need to establish their farms or improve their property.”The microloan program, which celebrates its third anniversary this year, has been successful, providing more than 16,800 low-interest loans, totaling over $373 million to producers across the country. Microloans have helped farmers and ranchers with operating costs, such as feed, fertilizer, tools, fencing, equipment, and living expenses since 2013. Seventy percent of loans have gone to new farmers.Now, microloans will be available to also help with farm land and building purchases, and soil and water conservation improvements. FSA designed the expanded program to simplify the application process, expand eligibility requirements and expedite smaller real estate loans to help farmers strengthen their operations. Microloans provide up to $50,000 to qualified producers, and can be issued to the applicant directly from the USDA Farm Service Agency (FSA).This microloan announcement is another USDA resource for America’s farmers and ranchers to utilize, especially as new and beginning farmers and ranchers look for the assistance they need to get started. To learn more about the FSA microloan program visit, or contact your local FSA office.last_img read more

World Cup 2011: Proteas look to weave spin web over NZ

first_imgSpin is expected to play a crucial, even dominant, role when South Africa clash with New Zealand in the third World Cup quarter- final here on Friday.South Africa captain Graeme Smith gave enough hints that he might include all three spinners – leg- spinner Imran Tahir, left- armer Robin Peterson and offie Johan Botha – in the XI, encouraged after watching Pakistan captain Shahid Afridi and other spinners snatch eight of the 10 West Indies wickets on Wednesday afternoon at the Sher- e- Bangla Stadium.Smith’s New Zealand counterpart Daniel Vettori is, however, relying on his top order batsman to deliver the goods in the knock- out game.Vettori’s confidence must have been boosted after big- hitter Brendon McCullum recovered from a knee injury to keep wickets too.Vettori’s troublesome knee, due to which he missed the matches against Canada and Sri Lanka, is better and he will return to lead the side again in the most important game so far for a team that has so far failed to meet expectations.Going by the captains’ views, it’s going to be a battle between South African spin – not to forget their fast bowlers – and New Zealand batting. Even Vettori can exploit the pitch if it continues to provide as much assistance as it did to Afridi and Mohammad Hafeez.While New Zealand will be battling to put behind their indifferent form in the league stage, South Africa will be looking to lay the ghosts of the past World Cups and shrug off the chocker’s tag once and for all.advertisement’ Chocker’ and ‘ jinxed’ are the words that have often described the South African teams over the years, since their reentry into international cricket in early 1990s, for their tendency to lose crucial matches. And these words are likely to keep reminding Smith and his players on Friday that they have another opportunity to prove their critics wrong.Smith – and premier all- rounder Jacques Kallis – would take motivation from Dhaka city where South Africa won a major ODI title in 1998, though the venue was Bangabandhu Stadium.In the final of the ICC Knock- Out Tournament, as the Champions Trophy was then called, Kallis bagged five wickets and scored 37 in the final against the West Indies to win the man- of- the- match and man- of- the- tournament awards.Going by the two teams’ performance in the league stage, South Africa start as favourite to win the day- night encounter and inch closer to that elusive World Cup. But history will count for nothing and it is the form of the day that will come into play.Both teams fared differently in the league, with South Africa topping Group B with 10 points and the Kiwis just managing to scrape through from Group A, finishing fourth with eight points. Significantly, South Africa lost only one match, to England, but that narrow defeat again raised the ‘ chocker’ issue.South Africa started with a sevenwicket win over the West Indies and demolished the Netherlands before running into England and losing by six runs in a nail- biting thriller.However, they recovered and won a close game against India and that result would have given them the much- needed confidence. They didn’t have difficulty in brushing aside Ireland and Bangladesh to ease into the quarter- finals.New Zealand began their campaign on a high note by thumping a hapless Kenya before Australia thrashed them. Vettroi’s side bounced back with a 10- wicket win over a weak Zimbabwe and surprised Pakistan and expectedly beat the lowly Canadian side.Although the Kiwis lost to Sri Lanka, they made it to the second stage as anticipated.South Africa may be in form now, but the local fans would support New Zealand as Smith’s team defeated Bangladesh to shut them out of the quarter- final race.last_img read more

Brickell and Baggio nominated for NSW Sports Awards

first_imgTwo Touch Football representatives have been named as finalists in the New South Wales Sports Federation 2013 Sports Awards. David Baggio is a finalist for the Official of the Year, while Gary Brickell will receive a Distinguished Long Service award for his services to the sport of Touch Football over the past 30 years. 2013 has been a great year for both Baggio and Brickell, with Baggio officiating at the 2013 Super Trans Tasman Series in New Zealand in February and named as the inaugural Rick Borg medallist, the Leading Referee at the 2013 National Touch League. He was also named as the Ian Matthew Medal winner for Referee of the Year at the 2013 New South Wales Touch Association (NSWTA) Referees Awards night in May. Brickell was recognised at the NSWTA Referees Awards night, being awarded for 30 years of service. Brickell was also recognised at the 2013 NSWTA Blues Dinner last weekend, being named the Southern Suns Administrator of the Year for his work with the Wollongong Touch Association.The New South Wales Sports Federation 2013 Sports Awards program provides an opportunity to celebrate outstanding achievements and contributions to sport in New South Wales in 2013. The Awards program consists of 13 categories, including athletes, teams, coaches, officials, organisations, events, media and Distinguished Long Service.The 2013 Sports Awards Dinner will be held at Allphones Arena on Thursday, 28 November 2013. For more information of the 2013 NSW Sports Awards Dinner, please visit Related LinksNSW Sports Awardslast_img read more

a month agoEx-Man Utd midfielder Hargreaves: Greenwood just like Van Persie

first_imgAbout the authorPaul VegasShare the loveHave your say Ex-Man Utd midfielder Hargreaves: Greenwood just like Van Persieby Paul Vegasa month agoSend to a friendShare the loveMason Greenwood has compared to Robin van Persie by former Manchester United midfielder Owen Hargreaves.Greenwood’s brilliant goal gave United a 1-0 win over Astana at Old Trafford on Thursday.And Hargreaves saw glimpses of former United striker van Persie in the 17-year-old.He said: “I think you have to take the positives on a night like this and the positives are that they kept a clean sheet and Mason Greenwood scored a stunning goal.”It reminded me a little bit of Robin van Persie, chopping inside onto that right foot and scoring a great goal.”I’m really happy for him because, as you saw (Bukayo) Saka for Arsenal, the night was all about the academies really.”They produced tonight for Arsenal and for Manchester United .” last_img read more